| November 25 2020 | Ludovic Dutoit and Alana Alexander |
With the age of high-throughput sequencing, several techniques have been developed to genotype thousands of markers across hundreds of individuals at relatively low-cost. One group of those methods, Genotyping-by-Sequencing (GBS) cuts down on the amount of the genome sequenced by using restriction enzymes to repeatedly sequence the same genomic locations across individuals. The thousands of loci sequenced can bring high resolution to population genomic or phylogenomic analyses at a fraction of the cost associated with whole genome sequencing.
This one day workshop is designed to show how to process GBS/RAD data through hands-on analysis using the Stacks software pipeline.
| Time | Lesson |
|---|---|
| 09:30-10:30 | Keynote Speaker: Dr. Alana Alexander |
| 10:30-11:00 | Data101: From raw data to individual samples files |
| 11:00-11:15 | Break |
| 11:15-12:15 | De-novo assembly without a reference genome |
| 12:15-13:00 | Lunch Break |
| 13:00-13:30 | De-novo assembly without a reference genome |
| 13:30-14:30 | Assembly with a reference genome |
| 14:30-14:45 | Break |
| 14:45-16:30 | Population genetics analyses |
| 16:30 | End of the day |